|CoverYourASP --> Troubleshooting --> Database connection errors|
Cannot open the file.
MSDN references: Q161333 Q183060 Q207525 Q166029 Q174943
You have a permissions problem. No question. Many have disputed this over long email threads, but it always comes down to a permissions problem!
The solution is to ensure that read/write permission has been granted to the Internet Guest User (IUSR_<machinename>). The steps to accomplish this are detailed in this article.